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學術活動
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學術報告——Single Cell Analysis of Yeast Aging by Modern Approaches
發表日期: 2018-11-09
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  報告人:Dr . Yi Zhang(章益博士) 

  主持人:鄭岷雪 

  報告時間Date: 2018119日(星期五)下午2:00 

  報告地點(Addr:A5-201 

  報告摘要(Abstract:  

  Aging is characterized by progressive loss of physiological and cellular functions, but the molecular basis of this decline remains unclear. In budding yeast Saccharomyces cerevisiae, aging can be assayed by at least two methods: chronological lifespan (CLS) and replicative lifespan (RLS). The yeast RLS assay measures how many daughter cells a mother can produce before it ceases dividing, and has been proposed as an assay to identify conserved pathways affecting the continued viability of dividing cells such as stem cells in humans. Here we explored how replicative aging affects transcriptional dynamics in yeast using single-cell RNA sequencing. We found that aging perturbed the total mRNA amount in the single cell level, and interfere with gene expression profile of core biological pathways. Increased cell to cell transcriptional variability and progressively deteriorated mutational signature was also observed during replicative aging in yeast. These discoveries shed light on a comprehensive understanding of the molecular mechanisms of aging and will direct us to find effective therapeutic strategies to prevent from aging and aging related diseases in human beings.  

  報告人簡歷(CV: 

  Dr. Yi Zhang (章益 博士) [email protected] 教育背景和學術經歷: 2015 10 至今: 北京大學生物動態光學成像中心博士后 1. 在黃巖誼教授實驗室從事博士后研究,并應用單細胞 RNA 測序技術研究酵母復制衰老。 2. 成功開發出一套針對微生物的單細胞轉錄組分析方法和技術,并且在世界上首次從單細胞水平對酵母衰老進行轉錄組分析和研究,進一步揭示了生物衰老的分子機制。 2013 9 2015 2 : 馬克思普朗克生物化學研究所(德國)博士后 1. Dr. Ulrich Hartl 教授實驗室從事博士后研究。 2. 應用單細胞成像技術和微流芯片技術研究 PolyQ 蛋白錯誤折疊和聚集相關的神經退行性疾病分子機制。 2010 10 2012 8 : 加州大學舊金山分校訪問學者 1. 加州大學舊金山分校生物化學和生物物理系訪問學者(訪問博士研究生)。 2. 應用單細胞成像技術和微流芯片技術在單細胞水平上研究酵母復制衰老的分子生物學機制。 2008 9 2013 7 : 北京大學物理學院博士研究生 1. 在歐陽頎院士實驗室攻讀生物物理方向博士研究生,開發微流芯片技術并用于酵母衰老研究。 2. 開發出一套高通量微流芯片系統,并應用于酵母衰老研究,革命性地改進了在單細胞尺度上對酵母復制壽命進行分析的方法。 Education & Academic Experiences: Oct.2015—Now: Peking University, Beijing, China 1. Post-Doctoral fellow in Dr. Yanyi Huang(黃巖誼教授) lab and conducted single cell RNAseq analysis of yeast replicative aging. 2. Successfully developed the approach for single cell transcriptome analysis in microorganism and applied on yeast aging research at the single cell resolution. Sep.2013—Feb.2015: Max Planck Institute for Biochemistry, Germany 1. Post-Doctoral fellow in Dr. Ulrich Hartl lab and conducted single cell analysis of PolyQ aggregation in yeast using microfluidic system. Oct.2010—Aug.2012: University of California, San Francisco, USA 1. Visiting PhD student in Department of Biochemistry and Biophysics, UCSF 2. Studied yeast replicative aging at the single cell resolution using the microfluidic system combined with single cell imaging. Sep.2008—July.2013: Peking University, Beijing, China 1. PhD student at School of Physics, Peking University majored in Biophysics and conducted microfluidics and aging research in Dr. Qi Ouyang(歐陽頎院士) lab. 2. Developed a high throughput microfluidic system for yeast aging research and revolutionized the approach of yeast replicative lifespan analysis at the single cell resolution.  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  文章發表: 1. Yi Zhang, Jiaxin Gao, Yanyi Huang, Jianbin Wang. Recent developments in single-cell RNA-seq of microorganisms. Biophysical Journal. (2018). 2. Zhengwei Xie, Kyle A. Jay, Dana L. Smith, Yi Zhang, Zairan Liu, Jiashun Zheng, Ruilin Tian,Hao Li, Elizabeth H. Blackburn. Early Telomerase Inactivation Accelerates Aging Independently of Telomere Length. Cell. (2015). 3. Yi Zhang, Chunxiong Luo, Ke Zou, Zhengwei Xie, Onn Brandman, Qi Ouyang, Hao Li. Single cell analysis of yeast replicative aging using a new generation of microfluidic device. PLoS One. (2012). 4. Susanne M. Rafelski, Matheus P. Viana, Yi Zhang, Yee-Hung M. Chan, Kurt S.Thorn, Phoebe Yam, Jennifer C. Fung, Hao Li, Luciano da F. Costa, Wallace F. Marshall. Mitochondrial Network Size Scaling in Budding Yeast is Achieved in the Bud at the Expense of the Mother. Science. (2012). 5. Zhengwei Xie, Yi Zhang, Ke Zou,Onn Brandman, Chunxiong Luo,Qi Ouyang, Hao Li. Molecular phenotyping of aging in single yeast cells using a novel microfluidic device. Aging Cell. (2012). 

  

  

  

  

  

  

  

  

  

  

  

  

  

  

  歡迎大家踴躍參加! 

   

  中國科學院生物醫學檢驗室重點實驗室 

  2018119 

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